作物学报2011，Vol.37Issue(11)：2117-2121,5.DOI:10.3724/SP.J.1006.2011.02117
以实时荧光定量PCR技术检测转基因玉米MON88017
Detection of Genetically Modified Maize MON88017 by Quantitative Realtime PCR
摘要
Abstract
Transgenic event-specific primers and Taqman probes based on the left-flanking sequence of MON88017 and endoge-nous gene (zSSIIb) were designed, and a real-time PCR method was developed to quantitatively detect the event-specific geneti-cally modified maize. The reference molecule composed of endogenous gene and flanking sequence of MON88017 was con-structed artificially. Two standard curves of the reference gene sequence and the 5' flanking sequence were established. We de-tected five mixed samples, and their genetically modified contents of MON88017 were 0.01%, 0.05%, 0.1%, 0.5%, and 1%, respectively. The lowest detection limit was 19-30 copies. This study indicated that the Taqman probes real-time PCR is highly specific and sensitive.关键词
转基因生物/实时荧光定量PCR/MON88017/标准分子Key words
Genetically modified organism/ Real-time PCR/ MON88017 event/ Reference molecule引用本文复制引用
袁磊,孙红炜,李凡,李宁,赵蕾,路兴波..以实时荧光定量PCR技术检测转基因玉米MON88017[J].作物学报,2011,37(11):2117-2121,5.基金项目
本研究由国家转基因生物新品种培育科技重大专项(2008ZX08012-001)和山东省农业科学院博士科研启动基金项目资助. （2008ZX08012-001）
