植物学报2012，Vol.47Issue(1)：44-54,11.DOI:10.3724/SP.J.1259.2012.00044
复苏植物旋蒴苣苔棉子糖合酶基因的克隆和表达
Cloning and Expression of a Gene Encoding a Raffinose Synthase in the Resurrection Plant Boea hygrometrica
摘要
Abstract
Resurrection plants are a valuable resource for understanding the mechanisms of dehydration tolerance and for mining candidate genes that can be used to improve drought tolerance in crops. Here, we determined the contents of galactinol, raffinose and stachyose in dehydrated and rehydrated leaves of a resurrection plant Boea hygrometrica. We cloned a dehydration-inducible gene encoding a raffinose synthase from B. Hygrometrica and found that BhRFS was upregulated by cold (4"C), high salt (200 mmol-L~1NaCI), and abscisic acid (ABA, 100 umol-L"1) but downregulated by high temperature (37°C) and not affected by H2O2 (200 umolL"1). BhRFS may function in response to various abiotic stresses, possibly regulated by ABA-dependent signal pathways, therefore it is a good candidate gene for plant breeding to enhance plant stress tolerance.关键词
旋蒴苣苔/棉子糖系列寡糖/棉子糖合酶/复苏植物Key words
Boea hygrometrica/ raffinose family oligosaccharides/ raffinose synthase/ resurrection plant引用本文复制引用
王智,刘永秀,魏建华,邓馨..复苏植物旋蒴苣苔棉子糖合酶基因的克隆和表达[J].植物学报,2012,47(1):44-54,11.基金项目
国家自然科学基金(No.30900106)和转基因生物新品种培育重大专项(No.2009ZX08009-060B) （No.30900106）
