植物研究2012，Vol.32Issue(1)：32-37,6.
黄芩种质资源ISSR遗传多样性的分析及评价
Analysis and Evaluation on Genetic Diversity of Scutellaria baicalensis G.by ISSR Markers
摘要
Abstract
To study the genetic diversity of Scutellaria baicalensis G. , 147 individuals from six populations were analyzed by inter-simple sequence repeat (ISSR). Eighteen primers were selected to produce highly reproducible ISSR bands. Among 485 amplified bands, 466 showed polymorphism, the percentage of polymorphic bands reached 96.08%. Nei' s gene diversity index and Shannon information index were 0.2444 and 0.388 9, respectively. Number of alleles (Na) and effective number of alleles (Ne) were 1. 993 8 and 1. 383 9, respectively. Gst was 0. 123 3 , genetic identity (I) and genetic distance (D) were 0.951 5 and 0.050 1, respectively. The results showed that the collected germplasm resources of S. Baicalensis had a high genetic diversity in general. There was a certain genetic differentiation and gene flow among different populations and the genetic variation was mainly within populations. Molecular clustering results showed that the same region germplasms were not clustered completely following the collection source. It may be related to folk culture introduction or less than normal for ISSR primers. The genetic diversity of S. Baicalensis germplasm resources can provide a basis for the evaluation , preservation and breeding of new varieties in the future.关键词
黄芩/种质资源/ISSR/遗传多样性/聚类分析Key words
Scutellaria baicalensis G. /germplasm/ISSR/genetic diversity/cluster analysis分类
农业科技引用本文复制引用
文苗苗,李桂双,张龙进,郑鹏,白成科..黄芩种质资源ISSR遗传多样性的分析及评价[J].植物研究,2012,32(1):32-37,6.基金项目
国家"十一五"科技支撑计划项目(2006BAI06A12-04)和陕两省科技计划项目(2011K16-02-05)基金支持 （2006BAI06A12-04）
