植物研究2012，Vol.32Issue(1)：61-68,8.
橡胶草HMGR基因的克隆及表达分析
Cloning and Expression Analysis of HMGR Gene from Taraxacum kok-saghyz
摘要
Abstract
Based on the design of degenerated oligonucleotides according to the conservative regions of nine 3-hydroxy-3-methylglutaryl-CoA reductases from nine plants and the total RNA extracted from Taraxacum kok-saghyz, a HMGR named TKHMGR was first obtained using the techniques of degenerate RT-PCR and RACE. Comparison of the amino acid sequence homology and system evolution analysis show that, TKHMGR belongs to the new members of the HMGR gene families. At the same time, the gene expression in different organizations was analyzed using fluorescence quantitative method. The cloning, bioinformatics analysis and expression analysis of this gene laid a foundation for further research on its function.关键词
橡胶草/HMGR/基因克隆/序列分析/荧光定量Key words
Taraxacum kok-saghyz (TKS) /3-hydroxy-3-methyl-glutaryl-coenzyme A reductase ( HMGR ) /gene cloning/sequence analysis/fluorescence quantitation分类
农业科技引用本文复制引用
王启超,刘实忠,校现周..橡胶草HMGR基因的克隆及表达分析[J].植物研究,2012,32(1):61-68,8.基金项目
中国热带农业科学院橡胶研究所基本业务专项(No.xjsz_06) （No.xjsz_06）
