植物研究2012，Vol.32Issue(1)：115-119,5.
光萼荷属植物SSR反应体系确立与指纹图谱构建
Establishment of SSR Reaction System and Fingerprinting for Some Aechmea Species
摘要
Abstract
The SSR reaction system was established by orthogonal design and the SSR fingerprints were constructed for some ornamental bromeliads in Aechmea in this study. The results showed that the optimal SSR reaction system of Aechmea was a total of 10 μL containing 1 xPCR buffer, Mg2+ 2. Ommol · L-1, dNTPs 200 pjnol · L-1, primers 2.5 μmol · L-1, template DNA 90 ng and Tag DNA polymerase 1.5 U. Three informative and polymorphic SSR primers, namely Ml, M3 and M4, were screened out from 6 SSR primers based on the established SSR reaction system. Three molecular fingerprints were preliminarily constructed for 15 samples of Aechmea species by these three SSR primers, respectively. The establishment of SSR fingerprint proved the SSR reaction system was stable and reliable, and could be further used in germplasm identification of Aechmea.关键词
光萼荷属/SSR/正交实验设计/反应体系优化/指纹图谱Key words
Aechmea/ SSR/ orthogonal experiment design/ optimization of reaction system/ fingerprint分类
农业科技引用本文复制引用
张飞,王炜勇,张智,田丹青,刘建新,刘晓静..光萼荷属植物SSR反应体系确立与指纹图谱构建[J].植物研究,2012,32(1):115-119,5.基金项目
国家自然科学基金(31101570) （31101570）
浙江省自然科学基金(Y3110271) （Y3110271）
浙江省重大科技专项(2009C12095) （2009C12095）
浙江省农业公益性技术应用研究计划(2000C22014) （2000C22014）
浙江省重点科技创新团队项目(2011 R50034) （2011 R50034）
杭州市种子种苗专项(20110332H15) （20110332H15）
浙江省农科院创新能力提升工程(2011R25Y01D01) （2011R25Y01D01）
