植物营养与肥料学报2011,Vol.17Issue(6):1515-1521,7.
一株具有ACC脱氨酶活性固氮菌的筛选与鉴定
Screening and identification of a nitrogen fixing bacteria with 1-aminocyclopropane-1-carboxylate deaminase activity
摘要
Abstract
ACC(1-aminocyclopropane-1-carboxylate) deaminase is a common characteristic enzyme in plant growth promoting bacteria(PGPB)in rencent years.Many ACC deaminase positive bacteria have the ability to increase plant resistence against environmental stresses like drought,flood,salinity and high temperature,therefore,screening and investigation on the ACC deaminase positive bacteria are important for agriculture.In this work,one strain designed as 7037 was screened.The results show that the ACC deaminase activity of the strain is 2.530 μmol/(h·mg),protein and the nitrogenase activity is C2H4 10.068 nmol/(h·mg),protein.The strain 7037 shows a relative extensive utilization for carbon sources and strong environmental adaptability,and is identified as Arthrobacter sp.The pot trial shows that the fresh weight of Chinese cabbage inoculated with the strain 7037 is increased by 139% compared with the no inoculation control.The strain 7037 is possible to be further developed as an excellent strain for microbial fertilizer production.关键词
生物固氮/ACC脱氨酶/植物抗逆性/植物促生细菌Key words
biological nitrogen fixation/ACC deaminase/plant stress resistance/plant growth promoting bacteria分类
农业科技引用本文复制引用
陈倩,胡海燕,高淼,徐晶,周义清,孙建光..一株具有ACC脱氨酶活性固氮菌的筛选与鉴定[J].植物营养与肥料学报,2011,17(6):1515-1521,7.基金项目
国家“863”项目 ()
中央级公益性科研院所基本科研业务费专项资金(2011-33)资助 ()