植物营养与肥料学报2012，Vol.18Issue(1)：210-217,8.
田菁茎瘤固氮根瘤菌对小麦种子侵染的促生作用及其在根系内的定殖
Promotion for wheat growth and root colonization after infecting wheat seeds with Azorhizobium caulinodans
摘要
Abstract
Common eight wheat cuhivars were selected and the sensitive cuhivars were screened out through gnoto- biotic condition test in the laboratory after infecting of the Azorhizobium caulinodans ORS571 （A. caulinodans） to wheat seeds. Infection method of seed soaking, the density of A. caulinodans and adding glucose are also studied, and the colonization of green fluorescence protein （GFP） - labeled A. caulinodans in the wheat seedling were detec- ted by fluorescence microscope. The results show that Xiaoyan22 is the most sensitive wheat cultivar to A. caulino- clans, in the gnotobiotic condition the root length and shoot height are increased by 17.04% and 8.37% respective- ly compared to the control, the optimum dilution proportion of A. caulinodans is 1.0 ×10^8/mL, and it is helpful for the infection to add 1 g/L glucose in A. caulinoclans PBS solution. With fluorescence microscope detection the result shows that GFP -labeled A. caulinodans infects from wheat seeding root hairs and lateral root fractures, and it can colonize in the vascular bundle of root. The field test shows that there is obvious promotion for wheat growth after infecting wheat cultivars seeds with A. caulinodans.关键词
田菁茎瘤固氮根瘤菌/小麦/侵染/促生作用Key words
Azorhizobium caulinodans/wheat/infection/growth promotion分类
农业科技引用本文复制引用
刘华伟,孙超,杨呼,林晓军,郭蔼光..田菁茎瘤固氮根瘤菌对小麦种子侵染的促生作用及其在根系内的定殖[J].植物营养与肥料学报,2012,18(1):210-217,8.基金项目
国家自然科学基金项目(30700489,31071870) （30700489,31071870）
陕西省科学技术研究发展计划项目(2010K02-12-2) （2010K02-12-2）
西北农林科技大学摹本科研业务费专项资金项目(QN2009067)资助. （QN2009067）
