中国中西医结合杂志2011，Vol.31Issue(11)：1502-1505,4.
电针刺激对高龄鼠氯胺酮麻醉认知功能及β-淀粉样蛋白抗体的影响
Effect of Electroacupuncture on the Cognitive Function and Plasma Antibodies against Beta-arnyloid Protein in Aged Rats with Ketamine Anesthesia
摘要
Abstract
Objective To study the effects of electroacupuncture (EA) on the changes of behavior after ketamine anesthesia, and changes of serum antibodies against beta-amyloid (Aβ) and Aβ protein in the hippocampus of aged rats, thus exploring the effects of EA on the cognitive dysfunction. Methods Thirty 14-month old SD rats were randomly divided into 3 groups, I. E. , the control group (Group A), the ketamine anesthesia group (Group B), and the EA + ketamine anesthesia group (Group C), 10 in each group. 50 mg/kg katemine was in-traperitoneally injected to rats in Group B and Group C, once daily for 7 successive days. EA was performed to rats in Group C from the 1st day of the experiment after rats awoke completely from anesthesia, twice daily for 7 successive days. Changes of the ratio of the swim time in the original platform quadrant to the total swim time and the escape latency phase were observed by Morris water maze. The peripheral blood was withdrawn by the end of the experiment. Serum anti-Ap antibody contents were detected using enzyme-linked immunosorbent assay (ELISA). The expressions of Ap in the hippocampus were detected using Westen blot. Results Long-term application of ketamine could lower aged rats' cognitive function. In the navigation test, the escape latency phase of rats in Group B was significantly prolonged (P<0. 01). On the 7th day of the experiment, the serum level of anti-Ap antibodies was lower in Group B than in Group A (P<0. 05), while the serum level of anti-Ap antibodies was significantly higher in Group C than in Group B (P<0. 01). On the 7th day of the experiment, the expression of Ap in the hippocampus was higher in Group B than in Group A ( P <0. 05). Conclusion EA could increase the contents of anti-Ap antibodies in aged rats with ketamine anesthesia, decrease the expression of Ap in the hippocampus, alleviate the deposition of Ap, thus improving rats' cognitive dysfunction.关键词
电针/后认知功能障碍/淀粉样蛋白/β淀粉样蛋白抗体Key words
electroacupuncture/ post-operative cognitive dysfunction/ amyloid protein/ beta-amyloid protein antibody引用本文复制引用
蒋殿宇,吕黄伟..电针刺激对高龄鼠氯胺酮麻醉认知功能及β-淀粉样蛋白抗体的影响[J].中国中西医结合杂志,2011,31(11):1502-1505,4.基金项目
辽宁省教育厅基金资助项目(No.2008834) （No.2008834）
