中国中西医结合杂志2011，Vol.31Issue(11)：1510-1513,4.
毒热平注射液对流感病毒性肺炎小鼠肺组织MMP-9及TIMP-1含量的影响
Effects of Dureping Injection on the Contents of MMP-9 and TIMP-1 in the Lung Tissue of Mice with Pneumonia of Influenza Virus Infection
摘要
Abstract
Objective To observe the effects of Dureping Injection on the contents of matrix metalloprotein-ase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in the lung tissue of mice with pneumonia of influenza virus infection. Methods Sixty-six ICR mice were randomly divided into the normal group, the model group, the low, middle, and high dose Dureping Injection groups (0.435, 0.870, and 1.740 mg/d, respectively), and the positive control group (Ribavirin, 2.500 mg/d), 11 in each. The pneumonia of mice with influenza virus infection model was established using influenza virus strain FM1. Mice were intraperitoneally injected with 0.3 mL FM1 starting from the infection day, once daily. Five days later mice were killed to calculate the lung index. The pathomorphological changes of the lung tissue were observed using routine HE stained sections. The contents of MMP-9 and TIMP-1 in the homoge-nate of the lung tissue were detected by ELISA double antibody sandwich method. Results Compared with the normal group, obvious inflammation occurred in the lung tissue of mice in the model group. The lung index, the content of MMP-9, and the value of MMP-9/TIMP-1 increased significantly in the model group (P<0.01), while the content of TIMP-1 was not significantly different (P>0. 05). Compared with the model group, the content of MMP-9 in the low and middle dose Dureping Injection groups, and the positive control group was significantly lowered (P<0.01). The content of TIMP-1 in the low, middle, and high dose Dureping Injection groups, as well as the positive control group significantly increased (P<0.01) and the value of MMP-9/TIMP-1 decreased (P<0.01). Conclusion Dureping Injection could alleviate the inflammatory injury of the lung tissue through decreasing the content of MMP-9, elevating the content of TIMP-1 in the lung tissue, and regulating the value of MMP-9/TIMP-1 of mice with pneumonia of influenza virus infection , thus alleviating the inflammatory injury of the lung tissue.关键词
毒热平注射液/流感病毒/基质金属蛋白酶-2,9/基质金属蛋白酶抑制物Key words
Dureping Injection/ influenza virus/ matrix metalloproteinase-9/ tissue inhibitor of matrix metalloproteinase-1引用本文复制引用
祁广见,顾立刚,彭桂英,殷胜骏,范新生..毒热平注射液对流感病毒性肺炎小鼠肺组织MMP-9及TIMP-1含量的影响[J].中国中西医结合杂志,2011,31(11):1510-1513,4.基金项目
国家自然科学基金资助项目(No.30772872) （No.30772872）
