中国中西医结合杂志2011，Vol.31Issue(11)：1514-1517,4.
丹酚酸B和延胡索乙素对大鼠心室肌细胞L-型钙通道的影响
Effect of Salvianolic Acid B and Tetrahydropalmatine on the L-type Calcium Channel of Rat Ventricular Myocytes
摘要
Abstract
Objective To observe the effects of the separate and joint use of salvianolic acid B (SalB) and tetrahydropalmatine (THP) on the L-type calcium channel of rat ventricular myocytes. Methods Single isolated ventricular myocytes of rats were obtained using acute enzymolysis separation. The current of the L-type calcium channel was recorded using whole-cell patch clamp technique. Changes of the current peak value of the calcium channel (the vertical distance between the peak value point after activation of the calcium electric current and the electric current track after complete inactivation) were observed before and after medication. Results The inhibition rate of using SalB (at the dose of 1, 10, and 100 μmol/L) alone on the current peak value of the calcium channel was respectively (25. 3 ±16. 4)% (n=4), (44. 6 ±24. 0)% (n=6), and (86. 0 ±20. 4)% (n = 4). That of using THP (at the dose of 10, 30, and 100 μmol/L) alone on the current peak value of the calcium channel was respectively (22. 2 ±6.4)% (n=5), (27. 4 ±1.6)% (n=3), and (51. 0 ±23. 0)% (n = 9). The inhibition potency of joint use of SalB (1 μmol/L) and THP (10 μmol/L) on the current peak value of the calcium channel was stronger than using SalB (1 μmol/L) alone or THP (10 μmol/L) alone, showing statistical difference (P<0. 05). Atropine hydrochloric acid (14 mmol/L) could reverse the inhibition of THP on the L-type calcium channel, while strengthening the inhibition of SalB. Conclusions Both SalB and THP showed inhibition on the L-type calcium channel of rat ventricular myocytes. They could generate synergistic effects. Besides, their action mechanisms for regulating the L-type calcium channel were different.关键词
丹酚酸B/延胡索乙素/L-型钙通道/膜片钳Key words
salvianolic acid B/ tetrahydropalmatine/ L-type calcium channel/ patch clamp引用本文复制引用
孟红旭,王宝,刘建勋..丹酚酸B和延胡索乙素对大鼠心室肌细胞L-型钙通道的影响[J].中国中西医结合杂志,2011,31(11):1514-1517,4.基金项目
国家自然科学基金重点资助项目(No.30830118) （No.30830118）
国家科技重大专项课题(No.2009ZX0903-0030) （No.2009ZX0903-0030）
