中国中西医结合杂志2011，Vol.31Issue(11)：1527-1530,4.
解毒祛瘀滋肾方对MRL/lpr狼疮小鼠肾组织GRα的调控作用研究
Study on the Regulation of Jiedu Quyu Zishen Recipe on Glucocorticoid Receptor α in the Renal Tissue of MRL/Ipr Mice
摘要
Abstract
Objective To explore the action mechanism of Jiedu Quyu Zishen Recipe (JQZR) on the signal transduction of glucocorticoid receptor α (GRα) in the renal tissue of MRL/lpr mice. Methods Thirty MRL/lpr mice were randomly divided into three groups, I. E. , the model group, the Western medicine group, and the Chinese medicine group, 10 in each. Besides, another 10 Kunming mice was taken as the normal control group. The pathological changes of the renal tissue were observed using HE staining. The expression of Gra was analyzed u-sing Real-time PCR and Western blot. The effects of JQZR on the binding power of Gra to cyclophilin A were detected using co-immunoprecipitation. Results The renal injury degree of MRL/lpr mice in the Western medicine group and the Chinese medicine group was alleviated. Compared with the model group, the relative quantitation of Gra mRNA and protein expressions in the renal tissue of mice in the Western medicine group decreased, while they increased in the Chinese medicine group, showing statistical difference (P<0. 01). JQZR could significantly elevate the binding potency of Gra to cyclophilin A. Conclusion Up-regulating the expression of Gra and enhancing mutual actions of Gra and cyclophilin A was one of JQZR's effects on improving the lesion of the renal tissue.关键词
解毒祛瘀滋肾方/糖皮质激素受体α/亲环素A/MRL/lpr小鼠Key words
Jiedu Quyu Zishen Recipe/ glucocorticoid receptor α/ cyclophilin A/ MRL/lpr mice引用本文复制引用
徐莉,季巾君,谢志军,温成平,范永升..解毒祛瘀滋肾方对MRL/lpr狼疮小鼠肾组织GRα的调控作用研究[J].中国中西医结合杂志,2011,31(11):1527-1530,4.基金项目
国家自然科学基金资助项目(No.81072764) （No.81072764）
国家"十一五"科技支撑计划资助项目(No.2006BAI04A10) （No.2006BAI04A10）
浙江省教育厅科研项目(No.Y200908682) （No.Y200908682）
中医内科临床创新团队项目(No.2011R09042-05) （No.2011R09042-05）
