中国中西医结合杂志2011，Vol.31Issue(12)：1619-1626,8.
慢性阻塞性肺疾病稳定期肺气虚证及其中药干预的血浆代谢组学研究
Plasma Metabonomlcs Studies on the Stable Phase Chronlc Obstructive Pulmonary Disease Patients of Fel-ql Deficiency Syndrome and the Chinese Materla Medica Intervention
摘要
Abstract
Objective By using the metabonomics method, to study the plasma metabonomics of the stable phase chronic obstructive pulmonary disease (COPD) patients of Fei-qi deficiency syndrome (FQDS), and of Chinese materia medica (CMM) intervention, thus exploring possibly existent biomarkers. Methods Forty stable phase COPD patients of FQDS were recruited as Group A. Ljuwei Buqi Capsule (LWBQC) was given to them as intervention. A healthy control group (Group B, 37 cases) was set up. The pulmonary function test was performed on patients in Group B and Group A before and after intervention. The plasma metabolites were detected using high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). Statistical data were analyzed using principal component analysis (PCA) and partial least squares (PLS). The original spectrum and data of plasma metabonomics were compared between the two groups. The whole spectrum amino acid metabonomics tests were performed in the two groups. Results Compared with Group B, the pulmonary function significantly decreased before intervention in Group A (P<0.05). The pulmonary function was more mildly improved after 30 days' treatment than before treatment, but still lower than it in Group B (P<0.05). The metabolic spectrum before treatment in Group A was significantly different from Group B, but showing regressive trend to Group B after treatment. Fifteen possible disease markers were found in COPD patients of FQDS. Results of the whole spectrum of amino acid metabolomics showed different features. Conclusions The changes of metabolic spectrum and amino acids could be found in the stable phase COPD patients of FQDS using plasma metabonomics, and potential markers could be detected. The intervention of the stable phase COPD patients of FQDS by Chinese medicine could brought positive changes in the metabolic profiling and amino acid metabolism.关键词
氏谢组学/慢性阻塞性肺疾病/稳定期/肺气虚证/六味补气胶囊Key words
metabonomics/ chronic obstructive pulmonary disease/ the stable phase/ Fei-qi deficiency syndrome/ Liuwei Buqi Capsule引用本文复制引用
刘志刚,李泽庚,彭波,章天寿,徐彬,张念志,童佳兵,杨程..慢性阻塞性肺疾病稳定期肺气虚证及其中药干预的血浆代谢组学研究[J].中国中西医结合杂志,2011,31(12):1619-1626,8.基金项目
国家自然科学基金资助项目(No.81270781) （No.81270781）
安徽中医学院青年科学研究基金资助项目(No.2010qn006) （No.2010qn006）
