中国中西医结合杂志2011，Vol.31Issue(12)：1645-1650,6.
经前期综合征肝气郁证大鼠海马雌激素受体α和β研究
Study on Estrogen Receptors α and β in the Hippocampus of Premenstrual Syndrome Model Rats of Gan-qi Depression Syndrome
摘要
Abstract
Objective To study the distribution pattern, the protein expressions, and changes of functional activities of estrogen receptor (ER) a and p in the hippocampus of premenstrual syndrome (PMS) rats of Gan-qi depression syndrome (GDS), and to find out corresponding effect targets of Jingqianshu Granule (JG), thus providing clues for exploring the pathogenesis of PMS of GDS and the mechanisms of JG. Methods SD rats were randomly divided into three groups, I. E., the normal group, the model group, and the medication group, 7 in each. Resident intruder stress was used to establish the model in the model group and the medication group. JG was given to rats in the medication group at the dose of 10 mL/kg by gastrogavage while modeling. Equal volume of sterilized water was given to rats in the model group and the normal group, once daily, for 5 successive days. Then the location, protein levels, and ligand-binding capacities of Eroc and Erp in the hippocampus of rats in three groups were detected using immunohistochemical assay, Western blot, and dextran-active carbon binding assay. Results There was no difference in the distribution pattern of Eroc and Erp in the hippocampus of the three groups. In aspects of protein levels and estrogen-binding capacities of Eroc and Erp in the hippocampus, CA1 and CA3 regions, they increased more obviously in the model group than in the normal group (P<0. 05), while they decreased more significantly in the medication group than in the model group (P<0. 05). Conclusion Higher estrogen levels and enhanced expressions and activities of Era and Erp in the hippocampus might be important mechanisms for PMS of GDS, which might also be the effect targets for JG.关键词
经前期综合征/肝气郁证/雌激素受体/海马Key words
premenstrual syndrome/ Gan-qi depression syndrome/ estrogen receptor/ hippocampus引用本文复制引用
高杰,宋春红,徐凯勇,郭英慧,张惠云,乔明琦..经前期综合征肝气郁证大鼠海马雌激素受体α和β研究[J].中国中西医结合杂志,2011,31(12):1645-1650,6.基金项目
国家重点基础研究发展计划(973计划)资助项目(No.2011CB505102) （973计划）
国家自然科学基金资助项目(No.30930110、30973688、81072721) （No.30930110、30973688、81072721）
中国博士后基金资助项目(No.20090451343) （No.20090451343）
山东省博士后基金资助项目(No.200803033) （No.200803033）
