中国中西医结合杂志2011，Vol.31Issue(12)：1674-1679,6.
洋葱中黄酮类提取物对血脑屏障透过及神经保护作用研究
Study of Ravanolds Extracted from Onion on the Blood-Brain Barrier Permeation and Neuroprotective Effects
摘要
Abstract
Objective To study the flavanoids extracted from onion on the blood-brain barrier (BBB) permeation , and their effects on primary cultured neuron cell proliferation and apoptosis of SD rats using ethanol reflux method. Methods The brain microvascular endothelial cells (BMVECs) were first successfully primary cultured. Then rats BMVECs and astrocytes (Acs) were co-cultured to establish the in vitro BBB model. The flavanoids were extracted from onion using ethanol reflux method. The model was verified by transmission electron microscopy (TEM) and trans-epithelial electric resistance (TEER). The flavanoids permeability was tested using high performance liquid chromatography (HPLC). Meanwhile, rat neuron cells were cultured and exposed to H2O2 and flavanoids. Their effects on the cell proliferation and apoptosis were observed using MTT assay. The injury of neuron DNAwas analyzed using single-cell gel electrophoresis (SCGE) and immunofluorescent assay. Results The in vitro BBB model was successfully established by TEM and TEER. Results of HPLC proved flavanoids extracts could effectively permeate the BBB with the permeability of 60. 58%. The extractive at 10 -20 pg/mL showed obvious inhibition on the apoptosis of neuron cells induced by H2O2 and attenuated the injury of neuron DNA. Conclusions The flavanoids extracted from onion ethanol reflux method could effectively penetrate the BBB. They also showed obvious inhibition on the H2O2 induced neuron cell apoptosis and DNA injury.关键词
血脑屏障/黄酮类物质/神经元细胞/细胞凋亡/DNA损伤Key words
blood-brain barrier/ flavanoids/ neuron cell/ cell apoptosis/ DNA injury引用本文复制引用
何丹,杜文婷,范雪娇,杨鸣鸣,所起凤,刘戟..洋葱中黄酮类提取物对血脑屏障透过及神经保护作用研究[J].中国中西医结合杂志,2011,31(12):1674-1679,6.基金项目
教育部博士点基金新教师项目(No.20070610124) （No.20070610124）
教育部留学回国人员基金项目(No.2008890-19-11) （No.2008890-19-11）
