中国中西医结合杂志2012，Vol.32Issue(1)：76-79,4.
蜕皮甾酮对H2O2诱导氧化损伤的人晶状体上皮细胞核因子κB p65表达的影响
Effects of Ecdysterone on the Expression of NF-κB p65 In H2O2 Induced Oxidative Damage of Human Lens Epithelial Cells
摘要
Abstract
Objective To study the effects of ecdysterone (ECR) on the expression of nuclear factor ( NF)-kB in H2O2induced oxidative damage of human lens epithelial cells (HLECs). Methods The cultured HLECs were divided into 5 groups, I.e., the control group, the H2O2 group, the p-estradiol (E2) group, the ECR group, and the pyrrolidine dithiocarbamate group (PDTC) group. The expression rate of NF-kB p65 in the HLECs were detected by flow cytometer (FCM). Results The expression of NF-kB p65 occurred in normal HLECs (9.53%). The expression rate of NF-kB p65 in the H2O2 group obviously increased (39.87%, P<0.01). The expression rate of NF-kB p65 in the PDTC group obviously decreased (5. 90%, P<0.01). The expression rates of NF-KB p65 in the ECR group (13.99%) and the E2 group (25.18%) ranged between the control group and the H2O2 group, but still lower than that of the H2O2 group (P<0.01). Conclusions The activation of NF-kB in the HLECs could be induced by H2O2 ECR with the estrogenic activity could effectively inhibit the activation of NF-kB.关键词
蜕皮甾酮/核因子κB/人晶状体上皮细胞/氧化损伤Key words
ecdysterone/nuclear factor-kappa B/human lens epithelial cell/oxidative damage引用本文复制引用
冯春燕,黄秀榕,祁明信,唐宋文,郭娜,胡艳红,陈胜,柯发杰..蜕皮甾酮对H2O2诱导氧化损伤的人晶状体上皮细胞核因子κB p65表达的影响[J].中国中西医结合杂志,2012,32(1):76-79,4.基金项目
福建省卫生厅青年科研课题(No.2009-1-30) （No.2009-1-30）
福建省教育厅课题(No.JA10176) （No.JA10176）
