中国中西医结合杂志2012，Vol.32Issue(2)：229-233,5.
阿魏酸对血管内皮细胞生长因子诱导的血管平滑肌细胞迁移的影响
Study on the Effects of Ferulic Acid on the Vascular Smooth Muscle Cell Migration in vitro
摘要
Abstract
Objective To investigate the effects of ferulic acid on the migration of vascular smooth muscle cells (VSMCs) and its correlated mechanisms. Methods VSMCs were in vitro cultured. Under the vascular endo-thelial growth factor (VEGF) induced conditions, the VSMCs migration were detected using the scratch test and the invasion assay after intervened by ferulic acid. The effects on the mRNA expressions of matrix metalloproteinase, (MMP)-2 and MMP-9 were detected using RT-PCR. The effects on the protein expressions of the tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2 were detected using Western blot. Results (1) Ferulic acid (102 ng/mL and 103 ng/mL) could inhibit VEGF-induced VSMCs migration. (2) Ferulic acid (102 ng/mL and 103 ng/mL) could down-regulate the VEGF-induced VSMCs migration by inhibiting MMP-9 mRNA expression. (3) Ferulic acid (102 ng/mL and 103 ng/mL) up-regulated VEGF-induced VSMCs TIMP-2 protein expressions. Conclusion Ferulic acid (102 ng/mL and 103 ng/mL) could inhibit VEGF-induced VSMCs migration by inhibiting the MMP-9 mRNA expression, and increasing the TIMP-2 protein expression.关键词
平滑肌细胞/阿魏酸/迁移/基质金属蛋白酶/金属蛋白酶组织抑制剂Key words
vascular smooth muscle cell/ ferulic acid/ migration/ matrix metalloproteinase/ tissue inhibitor of metalloproteinase引用本文复制引用
袁卓,张军平,杨萃..阿魏酸对血管内皮细胞生长因子诱导的血管平滑肌细胞迁移的影响[J].中国中西医结合杂志,2012,32(2):229-233,5.基金项目
国家自然科学基金资助课题(No.30973731) （No.30973731）
