中国中西医结合杂志2012，Vol.32Issue(2)：239-243,5.
紫草素对白血病细胞HL-60增殖及凋亡作用的影响
Effects of Shikonin on the Proliferation and Apoptosis of HL-60 Cells
摘要
Abstract
Objective To explore the mechanism of shikonin for inducing the apoptosis of human promye-locytic leukemia cell HL-60. Methods The effects of shikonin on the HL-60 cell proliferation were detected using MTT. The apoptosis rate was analyzed by Annexin-V/PI double staining. The expression level of the bcl-2 gene was detected using semi-quantitative reverse transcriptase PCR (RT-PCR), thus analyzing the correlation between the bcl-2 expression level and the apoptosis of HL-60. Results Shikonin could inhibit the proliferation of HL-60 cells with the concentration range of 1~ 8 μg/mL in a time- and concentration-dependent manner. Two μg/mL shikonin could induce the apoptosis of HL-60 cells in a time-dependent manner. The expression level of bcl-2 was obviously down-regulated at 2 |jg/mL shikonin. Conclusions Shikonin could induce the apoptosis of HL-60 cells. Its mechanism was correlated with down-regulation of the expression level of bcl-2.关键词
紫草素/HL-60细胞/细胞凋亡/bcl-2Key words
shikonin/ HL-60 cell/ apoptosis/ bcl-2引用本文复制引用
陈志炉,戴其舟,王勇,任莉,蒋慧芳,周郁鸿..紫草素对白血病细胞HL-60增殖及凋亡作用的影响[J].中国中西医结合杂志,2012,32(2):239-243,5.基金项目
浙江省中医药科技计划项目(No.2008CA025) （No.2008CA025）
浙江省医药卫生科技项目(No.2008B032) （No.2008B032）
