中国中西医结合杂志2012，Vol.32Issue(2)：244-247,252,5.
姜黄素对Bel7402/5-Fu细胞系多药耐药逆转作用的实验研究
Reversing Effects of Curcumin on Multi-drug Resistance of Bel7402/5-Fu Cell Line
摘要
Abstract
Objective To investigate the reversing effects of curcumin on hepatocellular carcinoma drug resistance Bel7402/5-Fu cell line. Methods Through the exposure to gradual increased concentrations of 5-fluoroura-cii (5-Fu), the cell line Bel7402 was induced to establish a multi-drug resistant sub-cell line Bel7402/5-Fu. The sensitivity to 6 chemotherapeutics of Bel7402 and Bel7402/5-Fu were detected using methyl thiazolyl tetrazolium (MTT) assay. The 50% inhibitory concentration (IC50) and resistant index (Rl) were calculated. The differences of the inhibition ratio of Bel7402/5-Fu by curcumin, 5-Fu, curcumin combined with 5-Fu were detected using MTT assay. The effects of curcumin, 5-Fu, curcumin combined with 5-Fu on the Bel7402/5-Fu apoptosis were detected using flow cytometry. Results The Bel7402/5-Fu cell line showed multi-drug resistance (MDR) to various chemotherapeutics, with the highest Rl shown of 5-Fu (being 109.55 ±14.30 times). The inhibition ratio of 5, 10, and 20 μg/mL curcumin combined with 5-Fu (50% IC50) was respectively 21.47% ±1.49%, 27.10% ±2.32%, and 59. 37% ±2.45%. The Bel7402/5-Fu apoptosis ratio of 5, 10, and 20 μg/mL curcumin combined with 5-Fu (50% IC50) was 30.92% ±2.10%, 44.87% ±2.24%, and 50.36% ±2.58%, respectively, which was obviously higher than that of the curcumin group and the 5-Fu group. Besides, the apoptosis rate increased along with increased curcumin concentration in the range of 0 -20 μg/mL. Conclusion Curcumin could induce the apoptosis of Bel7402/5-Fu. Meanwhile, it showed favorable reversing effects on MDR.关键词
姜黄素/多药耐药/肝癌Key words
curcumin/ multi-drug resistance/ hepatocellular carcinoma引用本文复制引用
曹仕琼,尹太勇,扬盛力..姜黄素对Bel7402/5-Fu细胞系多药耐药逆转作用的实验研究[J].中国中西医结合杂志,2012,32(2):244-247,252,5.基金项目
湖北省自然科学基金一般项目(No.2009CDB117) （No.2009CDB117）
