中药材2011，Vol.34Issue(11)：1656-1660,5.
滇杠柳茎段和顶芽组织培养及植株再生研究
Study on Tissue Culture and Plant Regeneration of the Stem-tips and Buds of Periploca forrestii
摘要
Abstract
Objective:To establish the rapid propagation systems of the stem-tips and buds of Periplocaforrestii. Methods:Insertedthe stem-tips and bud9 of Periploca fonestii into MS medium with different concentrations of 6-BA, NAA and 2.4-D and induced them growing into complete plants. Results and Conclusion: The optimal culture medium for bud induction is MS +6-BA 1.0 mg/L + NAA 0. 3 mg/L and the bud induction rate can reach 86. 29%. The optimal culture medium for stem-tips induction is MS +6-BA 2. 0 mg/L + NAA 0. 5 mg/L and the bud induction rate can reach 86. 29%. The optimal culture medium for bud multiplication is MS + 6-BA 2.0 mg/L + NAA 0. 1 mg/L and the multiplication coefficient can reach 2. 10. The best rooting medium is 1/2 MS + IBA 0. 5 mg/L and the rooting rate is 53. 33%.关键词
滇杠柳/组织培养/MS培养基Key words
Periploca forrestii Schlt/Tissue culture/MS medium分类
医药卫生引用本文复制引用
刘志飞,高洁,牛亚慧,石磊,陈涛涛..滇杠柳茎段和顶芽组织培养及植株再生研究[J].中药材,2011,34(11):1656-1660,5.基金项目
西南大学人才引进项目(207011) （207011）
重庆市科技攻关项目(CSTC,2008 AB1001) （CSTC,2008 AB1001）
