中药材2011，Vol.34Issue(11)：1678-1681,4.
甘肃产不同品种藏药绿绒蒿的质量评价方法研究
Study on Quality Control Methods for Different Varieties of Meconopsis of Gansu
摘要
Abstract
Objective: To establish the quantitative determination method and TLC identification of Meconopsis and study the Vis-UV spectrum character for fast identification. Methods; Meconopsis were identified by TLC, and the content of quercetin was determined by HPLC. Results: The acid hydrolyze condition was HCl-80% MeOH( 8: 25 )90 X. Calefaction by water for 1. 5 hour. The condition of HPLC : Hypersil-Keystone-C18(250 mm x4.6 mm,5 μm)column,MeOH-Water(50: 50) as mobile phase,detection wavelength at 360nm,flow rate at 1. 0 mL/min,column temperature at 35 °C .quercetin showed a good linear relationship at a range of 0. 01 ~0. 12μg,r = 0. 9997. The average recovery was 98. 83% , and RSD was 1. 69% (n = 9). The contents of quercetin in Meconopsis inte-grifolia and Meconopsis quintuplinervia of Gansu were 0. 1135 mg/g and 0. 0842 mg/g respectively. Conclusion; The method is simple, accurate and specific. It can be used for the quality control and fast identification of Meconopsis.关键词
绿绒蒿/槲皮素/酸水解/HPLC/TLC/可见-紫外光谱Key words
Meconopsis/ Quercetin/ Acid hydrolyze/ HPLC/ TLC/ Vis-UV分类
医药卫生引用本文复制引用
邵晶,郭玫,樊秦,程芳..甘肃产不同品种藏药绿绒蒿的质量评价方法研究[J].中药材,2011,34(11):1678-1681,4.基金项目
甘肃省中医药管理局基金项目(GZK-2008-31) （GZK-2008-31）
