中药材2011,Vol.34Issue(11):1722-1725,4.
顶空固相微萃取-气质联用法测定樟芝菌丝体中挥发性化合物
Analyze on Volatile Compounds of Antrodia camphorata Using HS-SPME-GC-MS
摘要
Abstract
Objective;To analyze the volatile compounds of Antrodia camphorata in solid-state and submerged cultures. Methods:A headspace solid-phase microextraction (HS-SPME) coupled with gaschromatography-mass spectrometry(GC-MS)were used to evaluate the profile of the volatile compounds. Results: 49 volatile compounds were identified in A. Camphorata mycelia in submerged culture, while 43 volatile compounds were identified in mycelia in solid-state culture. 1 -octen-3-ol, 3 -octanone, l-octen-3-ylacetate, acetic acid octyl ester and ethanol were the main volatile compounds in A. Camphorata mycelia in submerged culture, while 1-octen-3-ol, 3-oc-tanone,3-methyl-butyraldenhyde,y-podecalactone and methyl 2-furozte were the most potent key volatile compounds in mycelia in solid-state culture. Conclusion;The volatile compounds in the mycelia of A. Camphorata in solid-state and submerged cultures are similar but their relative contents are different关键词
樟芝/顶空固相微萃取/气相色谱-质谱/挥发性物质Key words
Antrodia camphorata/ Headspace solid-phase microextraction/Gas chromatography-mass spectrometry/Volatile compound分类
医药卫生引用本文复制引用
何喆,陆震鸣,许泓瑜,史劲松,许正宏..顶空固相微萃取-气质联用法测定樟芝菌丝体中挥发性化合物[J].中药材,2011,34(11):1722-1725,4.基金项目
江苏省自然科学基金(BK2010142) (BK2010142)
国家"863"重点项目子课题(2007 AA021506) (2007 AA021506)
教育部新世纪人才支持计划(NCET-07-0380) (NCET-07-0380)