中药材2011,Vol.34Issue(12):1940-1942,3.
翠云草总黄酮的提取工艺研究
Study on the Extraction Technology of Total Flavonoids from Selaginella uncinata
摘要
Abstract
Objective: To optimize the extraction technology of total flavonoids and improve the extraction ratio of total flavonoids from Selaginella uncinata. Methods; Using the content of flavonoids as the index determined by UV spectrophotometry, chose single factor experiment to investigate the total flavonoids content influenced by four factor ethanol concentration, extracting time, extraction times and solvent content on the influence of extraction efficiency. And on the basis, L9 (34) orthogonal experiment was carried out. Results: The ethanol reflux extraction conditions were as tollows: extracted in 60% ethanol (solid-liquid rate at 1: 35) for 3 times and heated for 1. 5 hours per time. Conclusion; The optimum extraction by the orthogonal design method is rational and feasible,provides experimental basis for improving the content of the total flavonoids and further scientific theoretical basis for industrial production of the total flavonoids from Selaginella uncinata.关键词
翠云草/总黄酮/提取工艺/正交设计Key words
Selaginella uncinata (Desv.)Spring/Total flavonoids/Ethanol reflux extraction/Orthogonal design分类
医药卫生引用本文复制引用
郑俊霞,郑扬,颜秋萍,钟新春,赵肃清,张焜..翠云草总黄酮的提取工艺研究[J].中药材,2011,34(12):1940-1942,3.基金项目
国家重点基础研究发展计划(973计划)(2006CB504100) (973计划)
广东工业大学校级博士科研启动项目(093002) (093002)