郑州大学学报(理学版)2011,Vol.43Issue(4):89-95,7.
太行山猕猴种群mtDNA遗传多样性研究
MtDNA-based Population Genetic Diversity of Rhesus Macaques in Mt.Taihangshan Area, Henan, China
摘要
Abstract
From November 2007 to February 2008, mtDNA-based population genetic diversity of rhesus macaques (Macaca mulatto tcheliensis) was investigated. Fecal samples were collected 5 separated groups among of 3 geographical units in Taihangshan Macaque National Nature Reserve (TMNNR) (35° ll'N, 112°16'E) in Henan, China. Total 22 mtDNA were extracted and used for genetic diversity analysis among different populations. The results showed that: ①the length of D-loop of mtDNA genome within 22 samples was 341 bp. Total 11 mutation sites, 9 of transition and 2 of trans version, were identified among 22 samples; ②there 9 haplotypes were found from 22 sequences within 3 geographical units; ③the results from AMOVA indicated that 88. 02% and 11. 03% of the genetic variation happened among groups and within populations, respectively, while there 0.95% of the genetic variations occurred within groups. There were significant genetic differentiation, while no shared haplotypes being found, among Jiyuan, Jiaozuo and Xinxiang geographic units (P < 0.01); and ④among the tested groups, Huanglianshu group in Jiyuan exhibited the highest haplotype number (3), the most haplotype diversity (0. 833) , the most nucleotide diversity (0. 002 93 ) and the most average number of nucleotide differences (1.000). The results implied that Huanglianshu group should be protected with priority in the point of genetic diversity.关键词
猕猴(Macaca mulatta)/线粒体DNA/遗传多样性/太行山Key words
rhesus macaques (Macaca mulatto)/ mtDNA/ genetic diversity/ Mt. Taihangshan分类
生物科学引用本文复制引用
谢东明,路纪琪,王建东..太行山猕猴种群mtDNA遗传多样性研究[J].郑州大学学报(理学版),2011,43(4):89-95,7.基金项目
国家自然科学基金资助项目,编号30770381 ()
郑州大学引进人才科研基金资助项目. ()