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太行山猕猴种群mtDNA遗传多样性研究

谢东明 路纪琪 王建东

郑州大学学报(理学版)2011,Vol.43Issue(4):89-95,7.
郑州大学学报(理学版)2011,Vol.43Issue(4):89-95,7.

太行山猕猴种群mtDNA遗传多样性研究

MtDNA-based Population Genetic Diversity of Rhesus Macaques in Mt.Taihangshan Area, Henan, China

谢东明 1路纪琪 1王建东2

作者信息

  • 1. 郑州大学生物多样性与生态学研究所 河南郑州450001
  • 2. 太行山猕猴国家级自然保护区济源管理局 河南济源454650
  • 折叠

摘要

Abstract

From November 2007 to February 2008, mtDNA-based population genetic diversity of rhesus macaques (Macaca mulatto tcheliensis) was investigated. Fecal samples were collected 5 separated groups among of 3 geographical units in Taihangshan Macaque National Nature Reserve (TMNNR) (35° ll'N, 112°16'E) in Henan, China. Total 22 mtDNA were extracted and used for genetic diversity analysis among different populations. The results showed that: ①the length of D-loop of mtDNA genome within 22 samples was 341 bp. Total 11 mutation sites, 9 of transition and 2 of trans version, were identified among 22 samples; ②there 9 haplotypes were found from 22 sequences within 3 geographical units; ③the results from AMOVA indicated that 88. 02% and 11. 03% of the genetic variation happened among groups and within populations, respectively, while there 0.95% of the genetic variations occurred within groups. There were significant genetic differentiation, while no shared haplotypes being found, among Jiyuan, Jiaozuo and Xinxiang geographic units (P < 0.01); and ④among the tested groups, Huanglianshu group in Jiyuan exhibited the highest haplotype number (3), the most haplotype diversity (0. 833) , the most nucleotide diversity (0. 002 93 ) and the most average number of nucleotide differences (1.000). The results implied that Huanglianshu group should be protected with priority in the point of genetic diversity.

关键词

猕猴(Macaca mulatta)/线粒体DNA/遗传多样性/太行山

Key words

rhesus macaques (Macaca mulatto)/ mtDNA/ genetic diversity/ Mt. Taihangshan

分类

生物科学

引用本文复制引用

谢东明,路纪琪,王建东..太行山猕猴种群mtDNA遗传多样性研究[J].郑州大学学报(理学版),2011,43(4):89-95,7.

基金项目

国家自然科学基金资助项目,编号30770381 ()

郑州大学引进人才科研基金资助项目. ()

郑州大学学报(理学版)

OA北大核心CSTPCD

1671-6841

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