组织工程与重建外科杂志2011，Vol.7Issue(6)：301-305,5.DOI:10.3969/j.issn.1673-0364.2011.06.001
毛乳头细胞和表皮细胞构建带附属器的组织工程皮肤
Reconstruction of Tissue Engineered Skin Substitute Containing Appendages Using Dermal Papillae Cells And Keratinocytes
焦虎 1范金财 1刘立强 1田佳 1甘承 1冯苏云 1付思祺 1陈文霖1
作者信息
- 1. 100144 北京市 中国医学科学院北京协和医学院整形外科医院
- 折叠
摘要
Abstract
Objective To reconstruct tissue-engineered skin substitutes containing appendages using human dermal papillae cells and keratinocytes together according to the developmental mechanism of the hair follicles. Methods Dermis substitutes of the experimental group were reconstructed by mixing both keratinocytes and dermal papillae cells into collagen gel. The collagen gel was seeded onto the surface of dermal substitutes. No cells were seeded onto the dermis in control group. The composite skin substitutes were cultured at the air-liquid interface for 5 days. A rounded full-thickness skin defect in diameter of lcm was made on the back of the nude mice and then covered with the skin substitute. The healing and contraction of wound was observed. At 4, 6, 8 weeks after transplantion, samples were obtained. Results The wound healing time was 2 and 4 weeks in experimental and control group respectively. The wound contraction was more greater in control group than in experimental group. Hair follicles and sebaceous glands formed in the dermis of the experimental group at 6 weeks after transplantation. Conclusion In corporation of dermal papillae cells and keratinocytes into dermal substitutes is a promising method to reconstruct tissue-engineered skin substitutes containing appendage.关键词
组织工程/皮肤替代物/毛乳头细胞/毛囊/皮脂腺Key words
Tissue engineering/Skin substitute/Dermal papillae cell/Hair follicle/Sebaceous gland分类
生物科学引用本文复制引用
焦虎,范金财,刘立强,田佳,甘承,冯苏云,付思祺,陈文霖..毛乳头细胞和表皮细胞构建带附属器的组织工程皮肤[J].组织工程与重建外科杂志,2011,7(6):301-305,5.
