组织工程与重建外科杂志2011，Vol.7Issue(6)：321-325,354,6.DOI:10.3969/j.issn.1673-0364.2011.06.006
负压创面治疗技术早期应用对软组织爆炸伤创面作用的研究
Effect of Vacuum-Assisted Infection Control and Granulation Formation on Soft Tissue Explosive Defects
摘要
Abstract
Objective To observe the effect of early NPWT application on infection control and granulation formation in explosive soft tissue defects by comparing the bacteria colony-forming unit and pathological changing. Methods Explosive soft tissue defects were created in pig model. Twelve defects were created on 3 pigs and divided into 2 groups randomly. NPWT group was on one side and Gauze group on opposed side. Each group had 6 wounds. Exposure group was composed of another 2 pigs with 6 wounds. At 6 hours, Day 3, Day 5 and Day 7, specimens were harvested in the center of the wound for counting of bacteria and HE staining. Microscopy was used for observation Granulation formation was observed by microscope. Results The numbers of bacteria colony on NPWT group were smaller than that of Gauze group and Exposure group at Day 3, 5 and 7. The counting of NPWT group peaked at Day 3 and declined below the critical value of infection at Day 5. After the NPWT application, the wound area shrank and became superficial. Compared with Gauze group, more granulation formation, and obvious staining with fibroblast and endothelium cell, as well as edema fading of the cells were observed in NPWT group. Conclusion Compared with the Gauze group, early application of NPWT can effectively control the bacteria multiplication and promote granulation formation on soft tissue explosive defects.关键词
爆炸伤/负压创面治疗/感染/肉芽Key words
Explosive injury/Negative pressure wound therapy/Infection/Granulation分类
医药卫生引用本文复制引用
钱玉鑫,芦立轩,侯强,江华,张文俊,刘安堂..负压创面治疗技术早期应用对软组织爆炸伤创面作用的研究[J].组织工程与重建外科杂志,2011,7(6):321-325,354,6.基金项目
全军医药卫生科研项目基金(08G069) （08G069）
南京军区医学科技创新课题(10MA026). （10MA026）
