安徽农业科学2012，Vol.40Issue(8)：4496-4498,4555,4.
产甘露聚糖酶菌株的筛选·鉴定及酶活性的初步研究
Preliminary Study on Selection and Identification of a Mannanase-producing Strain and Its Enzymatic Properties
摘要
Abstract
[Objective] This study aimed to identify a mannanase-producing strain isolated from soil. [ Method ] With konjac powder as the substrate, a mannanase-producing dominant strain was isolated from the soil samples collected from Kunyu Mountain by using plate selection method. Sequence analysis of the 16S rDNA fragment of the strain was conducted, and the strain was identified as Bacillus subtilis. Fermentation conditions and enzymatic characteristics were studied preliminarily. [Result] Experimental result showed that enzyme yield of this strain was different in different medium and in the same medium at different temperature. Enzyme yield of this strain in LB medium was higher when incubated at 27℃ than at 30℃ ; however, incubation at 30 ℃ was more conducive to the enzyme production than incubation at 27 ℃ in SOC medium. The optimal reaction pH was 7.0 and the optimal reaction temperature was 55 ℃ for enzyme production of this strain. When the temperature was above 55℃, enzyme activity declined sharply with the raise of temperature. Under the optimum conditions, enzyme activity could achieve 95. 3 U. [ Conclusion] This study provided a reference for the industrial application of degradation products of mannan.关键词
甘露聚糖酶/最适温度/最适pH/酶活力Key words
Mannanase/ Optimal temperature/ Optimal pH/ Enzyme activity分类
农业科技引用本文复制引用
武玉永,于敏,武芝..产甘露聚糖酶菌株的筛选·鉴定及酶活性的初步研究[J].安徽农业科学,2012,40(8):4496-4498,4555,4.基金项目
滨州医学院科技计划(BY2007KJ14). （BY2007KJ14）
