安徽农业科学2012，Vol.40Issue(8)：4544-4546,4574,4.
小花棘豆内生真菌中苦马豆素的鉴定及含量测定
Identification and Content Determination of Swainsonine of Endophytic Fungi from Oxytropis glabra
摘要
Abstract
[Objective] The aim was to explore whether endophytic fungi of Embellisia from Oxytropis glabra cultured in vitro produce swansoni-ne or not,and further study the relationship between the plant toxicity and endophytic fungi. [Method] The swainsonine of endophytic fungi from Oxytropis glabra was extracted by chemical method, and its protonated molecular ion structure was identified by MS. [ Result ] The results showed that all samples contained swasonine, and the m/z values of protonated molecular ions from all the samples and the swainsonine standards were 174. The content of swansonine was 24 -100 μg/g determined by GC. [ Conclusion] The study firstly found that endophytic fungi of Embellisia from Oxytropis glabra cultured in vitro produced swansonine, and the swansonine toxicity was related to the endophytic fungi.关键词
内生真菌/苦马豆素/质谱/气相色谱Key words
Endophytic fungi/ Swainsonine/ Mass spectrometry/Gas chromatography分类
农业科技引用本文复制引用
卢萍,霍红雁,钱亚光,李松,牛艳芳..小花棘豆内生真菌中苦马豆素的鉴定及含量测定[J].安徽农业科学,2012,40(8):4544-4546,4574,4.基金项目
国家自然科学基金项目(30860049). （30860049）
