安徽农业科学2012，Vol.40Issue(8)：4609-4611,3.
不同培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果研究
Proliferation of Avian Influenza Virus H9 Subtype in MDCK Cells in Different Culture Media
摘要
Abstract
[Objective]To screen the best culture media for proliferation of avian influenza virus ( AIV) H9 subtype in MDCK cells. [ Method] The MDCK monolayer cells were respectively cultured in DMEM containing 10% (V/V) newborn calf serum (NBS) ,low -serum culture medium (MEM - MD -611) and serum - free medium (SFE4Mega) and then inoculated with different dilutions of AIV H9 subtype. These three kinds of media containing 10 (Ag/ml trypsin were also used for maintenance culture of AIV. Every 24 h,cytopathic changes were observed and the HA titer of the culture supernatant was also determined. [Result] After 72 -96 h of culture,the HA titers of the culture supernatant derived from the serum - free medium, low - serum culture medium and serum containing medium were in a decreasing manner. [ Conclusion ] The DMEM containing 10% (V/V) newborn calf serum ( NBS),low - serum culture medium (MEM-MD-611) and serum-free medium (SFE4Mega) can be used for proliferation of AIV, and the latter two may be more suitable to prepare AIV culture.关键词
培养基/H9亚型流感病毒/MDCK细胞/增殖效果Key words
Culture medium/ Avian influenza virus H9 subtype/ MDCK cell/ Proliferation分类
农业科技引用本文复制引用
张建伟,史爱华,沈佳,景小冬,章振华,李林,姜北宇..不同培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果研究[J].安徽农业科学,2012,40(8):4609-4611,3.基金项目
北京市农林科学院一般项目(2010A007). （2010A007）
