安徽农业科学2012，Vol.40Issue(8)：4866-4867,4918,3.
鱼腥藻藻蓝蛋白的提取·分离纯化和抑菌研究
Study on the Extraction, Separation and Purification and Antibacterial Effect of Phycocyanin in Anabaena
摘要
Abstract
[Objective] The aim was to optimize the extraction of Anabaena phycocyanin, and study the separation and purification, gel electro-phoresis and bacteriostatic effects on crude phycocyanin. [Method] Using repeated freeze-thawing method to extract phycocyanin, and liquid chromatogram to separate and purify phycocyanin, SDS-PAGE electrophoresis to analyze protein component, moreover, using 96 orifice plate method culture and concentration measurement of enzyme standard instrument to study antibacterial effects. [ Result ] The optimum extraction conditions of phycocyanin are: solid to liquid ratio of 1:1, frozen time of 60 min, ammonium sulfate saturation of 60% . The maximum light-absorbing of phycocyanin is 620 nm. 3 kinds of proteins are obtained based on high performance liquid chromatography and marked as PC-1, PC-2, PC-3, respectively. SDS-PAGE electrophoresis show that the molecular weights of PC-1 and PC-2 are among 14 -18 Kd. PC-2 has weaker antibacterial action on Bacillus thuringiensis and Micrococcus luteus. [ Conclusion ] The study provided test basis for the extraction, purification and properties study of Anabaena phycocyanin.关键词
鱼腥藻/藻蓝蛋白/液相色谱/SDS-PAGE电泳/抑菌Key words
Anabaena/Phycocyanin/Liquid chromatogram/SDS-PAGE electrophoresis/Antibacterial分类
农业科技引用本文复制引用
苏明星,廖智,刘少华,李楠楠,王日昕,范美华..鱼腥藻藻蓝蛋白的提取·分离纯化和抑菌研究[J].安徽农业科学,2012,40(8):4866-4867,4918,3.基金项目
2010年浙江省大学生科技创新项目"鱼腥藻藻蓝蛋白提取工艺的优化和抗氧化、抗菌活性的研究" （）
浙江海洋学院重点项目(X09Z1) （X09Z1）
2011年浙江省大学生科技创新项目"海带多酚的提取优化和抑菌研究". （）
