安徽农业科学2012，Vol.40Issue(9)：5128-5129,2.
黄芪多糖单糖组分的气相色谱分析
Monosaccharide Composition in RADIX ASTRAGALI Polysaccharides by Gas Chromatography
摘要
Abstract
[ Objective ] To research on the composition of monosaccharide from RADIX ASTRAGALI polysaccharides. [ Method ] RADIX AS-TRAGALI polysaccharides sample was hydrolyzed with 2 mol/L sulfuric acid. Then, the dried hydrolysate was dissolved in pyridine, and deri-vatization was conducted by trimethylsilyl ether. Finally, the composition of monosaccharide from RADIX ASTRAGALI polysaccharides was detected by Capillary Gas Chromatography. [ Result] Arabinose ,fructose, glucose, mannose were identified from the RADIX ASTRAGALI pol-ysaecharides, And their molar ratio calculated according to the equation was 1:10.309:24. 667:0.462. Besides, three unknown matters were detected, with the retention time being 10. 627, 14. 783 and 18. 249 min. [ Conclusion] This method was simple and sensitive, and could be used for the component analysis of monosaccharide in RADIX ASTRAGALI polysaccharide.关键词
气相色谱/黄芪多糖/单糖组成Key words
Gas chromatography/RADIX ASTRAGALI polysaccharides/ Monosaccharides composition分类
农业科技引用本文复制引用
姚丹,王宏军..黄芪多糖单糖组分的气相色谱分析[J].安徽农业科学,2012,40(9):5128-5129,2.
