安徽农业科学2012，Vol.40Issue(9)：5150-5151,2.
RP-HPLC-ELSD法测定白花丹野生药材根·茎·叶中胡萝卜苷的含量
Content Determination of Daucosteroi in Roots, Stems and Leaves of Wild Plumbago zeylanica L.by RP-HPLC-ELSD
摘要
Abstract
[ Objective ] To establish a method for determining the content of daucosterol and compare its content from roots, stems and leaves of wild Plumbago zeylanica L.. [ Method ] High performance liquid chromatography - evaporative light scattering detection ( RP - HPLC -ELSD) was used to determine the content of daucosterol in wild P. Zeylanica. Diamondsil C18 (250 mm ×4.6 mm, 5 μm) was used with pure methanol as the mobile phase and the flow rate was 1.0 ml/min. The temperature of column was 25 ℃. The flow rate of nitrogen at 2.5 L/min and the temperature of drift tube was 65 ~C. [ Result ] The calibration curve of daucosterol was linear with concentration in.the range of 0. 129 to 2.58 μg/ml with r = 0. 999 4 ( n = 7 ). The average recovery was 99.98 % with RSD of 0. 212% ( n = 9). The content of daucosterol was the highest in leaves and lowest in roots. For each part, the content of daucosterol in P. Zeylanica was the highest in February, July, August,and the lowest in May and June. [ Conclusion] The method is rapid, simple, stable. And the results provide data for establishing the cultivat-ed technique of Plumbaginis zeylanicae L..关键词
白花丹/胡萝卜苷/含量测定/反相高效液相色谱法-蒸发光散射检测法Key words
Plumbago zeylanica L. / Daucosterol / Content determination / RP - HPLC - ELSD分类
农业科技引用本文复制引用
左旭,戴先芝,张吉仲,刘圆..RP-HPLC-ELSD法测定白花丹野生药材根·茎·叶中胡萝卜苷的含量[J].安徽农业科学,2012,40(9):5150-5151,2.基金项目
四川省杰出青年学术技术带头人后续计划(2011JQ0051),四川省科技支撑计划(2011SZ0233) （2011JQ0051）
中央高校基本科研业务费专项资金项目优秀科研团队及重大孵化项目(11NZYTH02) （11NZYTH02）
阿坝师专校级重点课题(ASA11-31). （ASA11-31）
