安徽农业科学2012，Vol.40Issue(9)：5160,5198,2.
HPLC法测定复方罗布麻颗粒中槲皮素的含量
Determination of Quercetin Content in Fufang Luobuma Granule by RP-HPLC
孙仁爽 1梁廷2
作者信息
- 1. 通化师范学院,吉林通化134000
- 2. 吉林紫鑫药业股份有限公司研发中心,吉林柳河135300
- 折叠
摘要
Abstract
[ Objective] To determine the content of quercetin in Fufang Luobuma granule. [ Method] RP - HPLC was adopted to detect the content. Chromatographic column was Zorbax-C18(250 mm × 4. 6 mm,5 μm) ; mobile phase was anhydrous methanol -0. 5% phosphoric acid ( V/V, 50:50) ; flow rate was 1.0 ml/min; detection wavelength was 370 nm; column temperature was 30 ℃ ; and injection volume was 10 μl. [Result] The average recovery rate of quercetin was 100. 2% and the RSD was 1. 94% (n =5). The quercetin content in Fufang Luobuma granule (sugar) was 0. 729 5 mg/g, and that in Fufang Luobuma granule (sugar free) was 0. 955 mg/g. [ Conclusion] This method was simple, accurate, and repeatable, and could be used for the content determination of quercetin in Fufang Luobuma granule.关键词
复方罗布麻颗粒/槲皮素/HPLC/含量测定Key words
Fufang Luobuma granule/ Quercetin/ HPLC/ Content determination分类
农业科技引用本文复制引用
孙仁爽,梁廷..HPLC法测定复方罗布麻颗粒中槲皮素的含量[J].安徽农业科学,2012,40(9):5160,5198,2.
