安徽农业科学2012，Vol.40Issue(9)：5196-5198,3.
反相HPLC法测定重庆不同产地野生天麻和栽培天麻的天麻素含量
Determination of Gastrodine Contents in Wild and Cultivated Gastrodia elata from Different Regions of Chongqing by RP-HPLC
黄娇 1姜登军2
作者信息
- 1. 重庆三峡医药高等专科学校,重庆万州404120
- 2. 重庆万州药检所,重庆万州404040
- 折叠
摘要
Abstract
[ Objective ] To compare the quality of wild and cultivated Gastrodia elata from different regions of Chongqing. [ Method ] Gastrod-ine was extracted from G. Elata with ultrasonic method and then determined by RP-HPLC with the acetonitrile-0.05% phosphoric acid (3: 97, V/V) as mobile phase at 1.0 ml/min,Diamonsil C18(4. 6 mm × 250 mm,5 μm) as the chromatography column,wavelength at 220 nm and column temperature at 30 ℃. [ Result] The gastrodine contents were greatly different in the G. Elata sample from different regions. The content of gastrodine in wild G. Elata was generally higher than that in the cultivated. The highest content of gastrodine was found in the cultivated G. Elata in Chengkou and the wild G. Elata in Yunyang,while the lowest content was found in the cultivated G. Elata in Wushan. [Conclusion] The content of gastrodine in both the wild and cultivated G. Elata in Chengkou was far above the national standards, therefore it was feasible to build a G. Elata planting base in Chengkou according to the national GAP requirements so as to satisfy the market needs.关键词
天麻(Gastrodia elata Bl.)/天麻素/反相高效液相色谱法/野生/栽培/含量Key words
Gastrodia elata/ Gastrodine/ RP-HPLC/ Wild/ Cultivated/ Content分类
农业科技引用本文复制引用
黄娇,姜登军..反相HPLC法测定重庆不同产地野生天麻和栽培天麻的天麻素含量[J].安徽农业科学,2012,40(9):5196-5198,3.
