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复合酶法制备马铃薯微孔淀粉的工艺研究

杨圣岽 李兵 张琳 李欣欣 张凌 曲波 王茜 王丽华

安徽农业科学2012,Vol.40Issue(9):5352-5354,3.
安徽农业科学2012,Vol.40Issue(9):5352-5354,3.

复合酶法制备马铃薯微孔淀粉的工艺研究

Study on Process Conditions of Preparation of Microporous Potatoes Starch with Complex Enzyme Method

杨圣岽 1李兵 1张琳 1李欣欣 1张凌 1曲波 1王茜 1王丽华1

作者信息

  • 1. 吉林大学生物与农业工程学院,吉林长春130000
  • 折叠

摘要

Abstract

[ Objective ] The effects of using α-amylase and glucoamylase to prepare microporous potato starch were studied. [ Method ] Taking potato starch as raw materials, starch hydrolysis rate and the oil absorption as a measure of index, the influences of the reaction temperature, two enzymes proportion, the quantity of enzyme, the chroma of substrate, buffer solution pH and reaction time on microporous potato starch were investigated. [ Result ] The experimental results showed that the best technological conditions were reaction temperature 45 ℃ , enzyme ratio (glucoamylase /a-amylase) 6, the quantity of enzyme 1.0% , the substrate quantity chroma 0.14 g/ml, buffer solution pH 4, the reaction time 8 h. In such process condition, the oil adsorption rate of hydrolyzed potato starch was as high as 70.2% , starch hydrolytic ratio was 34. 16%. [ Conclusion ] The study provided a basis for the development and utilization of microporous starch.

关键词

微孔淀粉/水解率/油脂吸附率/制备工艺/复合酶法

Key words

Microporous starch/ Hydrolysis rate/ Oil absorption rate/ Preparation craft/ Complex enzyme method

分类

农业科技

引用本文复制引用

杨圣岽,李兵,张琳,李欣欣,张凌,曲波,王茜,王丽华..复合酶法制备马铃薯微孔淀粉的工艺研究[J].安徽农业科学,2012,40(9):5352-5354,3.

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