安徽农业科学2012，Vol.40Issue(9)：5359-5360,5589,3.
固相萃取-高效液相色谱法测定蜜源生物液中3种四环素族抗生素的残留
SPE-HPLC Determination of Tetracycline Antibiotics Residues in Honey-original Biological Fluids
摘要
Abstract
[ Objective ] The aim was to establish a reliable method for the simultaneous determination of residues for oxytetracycline (OTC ) , tetracycline (TC) and chlortetracycline (CTC) by solid-phase extraction with high-performance liquid chromatography (SPE-HPLC) in honey-original biological fluids. [Method] 0.1 mol/L Na2EDTA-Mcllvaine solution was used as extraction solvent. After cleaning by Oasis HLB SPE column, TCs were separated by the mobile phase which included 0. 01 mol/L oxalic acid, acetonitrile, methanol, and the separation mode was gradient elution. [ Result] OTC and TC showed a good linear relationship in the range of 0.2 -20. 0 μg/ml, and the correlation coefficients were 0.999 6-0.999 8, CTC showed a good linear relationship in the range of 0. 5 - 20. 0 μg/ml, and the correlation coefficients was 0.999 8, and the average recoveries (n = 5 ) of the three compounds concerned were 98.4% - 104.9% . And the relative standard deviations were 1.9% -5.0%. [Conclusion] The method is simple, reliable, sensitive and suitable for the detection of the residuals for TCs in honey-original biological fluids.关键词
蜜源生物液/四环素族/固相萃取/高效液相色谱法Key words
Honey-original biological fluids/ TCs/ SPE/ HPLC分类
农业科技引用本文复制引用
张一,郝小燕,肖海涛,杨俊,章嵘..固相萃取-高效液相色谱法测定蜜源生物液中3种四环素族抗生素的残留[J].安徽农业科学,2012,40(9):5359-5360,5589,3.基金项目
贵州省科技厅工业攻关项目(黔科合GY字[2009]3053). （黔科合GY字[2009]3053）
