安徽农业科学2012，Vol.40Issue(9)：5363-5365,5630,4.
谷物中柠檬黄和日落黄高效液相色谱检测方法的优化
Optimization of the Test of Tartrazine and Sunset Yellow in Cereals by Means of HPLC
摘要
Abstract
[ Objective ] The aim was to explore an optimized method to measure the artificial pigment by means of high performance liquid chromatograph. [ Method] The gradient elution technique of HPLC was used to isolate and analyze tartrazine and sunset yellow in the cereals. The pre-treatment temperature, ultrasonic time, the column temperature and the elution conditions were studied, and the best experimental conditions were confirmed. [Result] At 40 ℃ , the optimal chromatography analysis condition was moving phase, with pH (6. 8) , methanol/ water of acetic acid-ammonium acetate (0.02 mol/L) , and column temperature (35 ℃ ). Gradient elution condition was 20% -35% methanol, 3%/min; 35% -98% , 6%/min; 98% , 8 min. The content of tartrazine and sunset yellow in cereal was tested precisely by the means of HPLC. The average recoveries of standard addition were 97. 9% and 100. 2% , and the detection limit were 0.02 mg/kg and 0.03 mg/kg. [ Conclusion ] This method shortened the analysis time, improved the detection sensitivity and the resolution of artificial color.关键词
谷物/柠檬黄/日落黄/高效液相色谱/优化Key words
Cereals/ Tartrazine/ Sunset yellow/ High performance liquid chromatography/ Optimization分类
农业科技引用本文复制引用
初红涛,张晖,王军,靳凤龙,苏立强..谷物中柠檬黄和日落黄高效液相色谱检测方法的优化[J].安徽农业科学,2012,40(9):5363-5365,5630,4.基金项目
黑龙省自然科学基金项目(B200911) （B200911）
齐齐哈尔大学青年教师科研启动支持计划项目(2010k-M17) （2010k-M17）
齐齐哈尔市工业攻关项目(GYGG2010-06-1). （GYGG2010-06-1）
