重庆医学2012，Vol.41Issue(11)：1043-1046,封2,5.DOI:10.3969/j.issn.1671-8348.2012.11.002
新生小牛皮肤的形态学和生物力学特点及脱细胞方法探索
Study on morphologic and biomechanical properties of newborn bovine skin and method for decellularization
摘要
Abstract
Objective To compare the dermal tissue structure ,content of difference types of collagen and biomechanical properties between human and newborn bovine skin ,and to find the evidence of bovine dermal matrix used in clinic ,in order to explore the decellularization of newborn bovine skin .Methods The dorsal full-thickness of samples were stained by hematoxylin and eosin (HE) staining ,Masson's trichrome staining ,sirius red staining ,and Gomori staining,and were examined under light microscopy . These samples were also observed by scanning electron microscopy (SEM ) and transmission electron microscopy (TEM ) .The relevant data of histological images were measured with image analysis software.The biomechanical properties of those samples were detected by material testing machine .And the dermal matrix was decellularized using trypsin combined detergent .Results Compared with human skin ,the thickness of the dermis and epidermis were significantly thinner ,and the ratio between the dermis and epidermis was significantly reduced in the newborn bovine skin.The gap rate between bundles [(41 .72+1.81) vs (40 .66+1.40)] and the caliber of the bundles [(11.28+0 .18)μm vs (10 .88+0 .66)μm] were not significant in statistics (P=0 .467 ,P=0 .368) . From the observation of scanning electron microscopy (SEM) examination,the dermal collagen bundles of newborn bovine were thinner and rarefaction arranged .Compared with human dermis ,the newborn bovine had longer cyclical transverse striation ,and the collagenous fibrils had no significant difference by transmission electron microscope (TEM ) .The maximum stress of newborn bo-vine[(21.08+0 .91) Mpa] was stronger than that of human dermis [(12 .76+1 .60) Mpa,P=0 .001],and the elastic modulus were also stronger than that of human [(82 .12+1.23) Mpa vs (48 .63+5 .50) Mpa,P=0 .001].The exploration of decellularization showed that according to the higher concentration of typsin and the longer of time ,the nucleus were more and more less ,and the destruction of dermal fiber were more and more big .Conclusion The dermis of newborn bovine and human were similar in fiber three dimensional structure .Although there have a difference in biomechanical properties ,the decellularization dermal matrix can used as biomaterials for reparing the human dermis .Combination appropriate concentration and time of trypsin can be prepared an Ideal bovine acellular dermal matrix.关键词
皮肤/组织学/生物力学/脱细胞真皮基质Key words
skin/ histology/ biomechanical properties/ decellularized dermal matrix引用本文复制引用
陈博,彭代智,左海斌,郑必祥,周灵..新生小牛皮肤的形态学和生物力学特点及脱细胞方法探索[J].重庆医学,2012,41(11):1043-1046,封2,5.基金项目
国家高技术研究发展计划(863计划)课题资助项目(2006AA02A121) （863计划）
国家重点实验室自主研究课题资助项目(SKLZZ200904). （SKLZZ200904）
