重庆医学2012，Vol.41Issue(11)：1055-1057,1061,4.DOI:10.3969/j.issn.1671-8348.2012.11.005
PI3K/Akt-NO信号通路在Ang Ⅳ诱导大鼠心肌肥大中的作用
Effect of PI3K/Akt-NO signal pathway on cardiomyocyte hypertrophy induced by angiotensin IV in rats
摘要
Abstract
Objective To study the effect of PI3/Akt-NO signal transduction pathway on cardiomyocyte hypertrophy induced by angiotensin IV (Ang IV ) .Methods The cardiomyocyte hypertrophy responses were assayed by measuring the cell surface area and atrial natriuretic factor mRNA expression in cultured neonatal rat cardiomyocytes .NO concentration and endothelial NO syn-thase (eNOS) activity were determined by using the nitrate reduction and ELISA methods ,respectively .The mRNA and protein expressions were detected by real-time PCR and western blotting respectively .In cultured cardiomyocytes ,LY294002 ,a selective PI3K antagonist ,and L-NAME ,a selective NOS antagonist ,were used to investigate the mechanisms of Ang IV .Results In cultured cardiomyocytes,Ang Ⅳ (0 .01,0 .1 ,1 nmol/L) induced cardiomyocyte hypertrophy in a concentration-dependent way .Meanwhile ,the expressions of Akt mRNA and protein decreased ,and the expression of eNOS mRNA and the concentrations of eNOS and NO reduced (P＜0 .05) .LY294002 and L-NAME enhanced these effects of Ang Ⅳ (P＜0 .05) .Conclusion PIsK/Akt-NO signal transduction pathway involves in cardiomyocyte hypertrophy induced by Ang Ⅳ,at least partly .关键词
血管紧张素Ⅳ/PI3K/Akt信号通路/心肌肥大Key words
angiotensin Ⅳ/ PI3 K/Akt/ signal pathway/ cardiomyoeyte hypertrophy引用本文复制引用
彭晓凤,陈加飞,王全华,周龙洋,王佳,蒋青松..PI3K/Akt-NO信号通路在Ang Ⅳ诱导大鼠心肌肥大中的作用[J].重庆医学,2012,41(11):1055-1057,1061,4.基金项目
国家自然科学基金资助项目(NSFC,81100905) （NSFC,81100905）
高等学校博士学科点专项科研基金资助项目(20105503120005). （20105503120005）
