重庆医学2012，Vol.41Issue(12)：1194-1196,3.DOI:10.3969/j.issn.1671-8348.2012.12.019
PCNA蛋白突变体的真核表达载体构建及鉴定
Construction and identification of eukaryotic expression vector for mutant PCNA
摘要
Abstract
To construct and express FLAG-PCNA gene mutant in 293T cells. Methods Mutant FLAG-PCNA cD-NA was amplified by PCR site-directed mutagenesis with FLAG-PCNA gene as template, and inserted into the eukaryotic expression vector pCMV-N-FLAG. The recombinant vector was then transfected into human embryonic kidney (HEK) 293T cells. The cells were harvested after 48h to identify the expression of plasmids by western blot. Results Sequences of recombinant plasmid were correct,and the mutants of PCNA were obtained. Conclusion Eukaryotic plasmids of mutant PCNA were obtained, and the expression of mutant PCNA protein was identified, which lay a foundation for further research of PCNA protein.关键词
PCNA蛋白/突变体/真核表达Key words
PCNA/mutant/eukaryotic expression引用本文复制引用
刘小会,淡松松,秦焕焕,高学娟,刘朗夏..PCNA蛋白突变体的真核表达载体构建及鉴定[J].重庆医学,2012,41(12):1194-1196,3.基金项目
国家973重点基础研究发展计划资助项目(2011CB910701) （2011CB910701）
国家自然科学基金青年科学基金资助项目(31000628) （31000628）
暨南大学引进优秀人才科研启动基金资助项目(50624001) （50624001）
广东省高等学校高层次人才资助项目(50524006). （50524006）
