重庆医学2012，Vol.41Issue(13)：1249-1251,封2,4.DOI:10.3969/j.issn.1671-8348.2012.13.001
水通道蛋白-4在大鼠创伤性脑水肿中的表达及其意义
Expression and its significance of aquaporin-4 in rat brain injury model
摘要
Abstract
Objective To investigate the changes of aquaporin-4(AQP4) expression after traumatic brain injury(TBI)in rats and its correlation with cerebral edema. Methods Healthy Wistar rats were randomly divided into the control group(n = 15)and the experimental group(n = 60). The experimental group adopted the improved Feeney method for establishing local TBI model of right cerebral hemisphere. The control group received the scalp incision without cerebral injury. The morphological observation of cerebral tissues and the brain water content(BWC)determination were performed. AQP4 expression was detected by the immunohisto-chemistry(IHC)method. Results The percentage of BWC in cerebral injury area at 72 h in the experimental group was[(80. 18 + 0. 55) %] , which was higher than[(78. 10 + 0. 52) %] in the control group(P<0. 05). The average absorbance value at 1,3 h in the experimental group were(0. 210 0 + 0. 029 4) ,(0. 186 0 + 0. 016 5) , which were higher than(0. 143 3 + 0. 053 8)in the control group (P<0. 05). Conclusion At the earlier stage of TBI, the expression of AQP4 is strongly increased, which may be related with the stress reaction after TBI. At the later stage of TBI,the expression of AQP4 is down-regulated and its activity is reduced,resulting in mixed cerebral edema.关键词
水通道蛋白-4/脑水肿/脑损伤/大鼠Key words
aquaporin-4 /brain edema/brain injuries/rats引用本文复制引用
陈通,孙善全..水通道蛋白-4在大鼠创伤性脑水肿中的表达及其意义[J].重庆医学,2012,41(13):1249-1251,封2,4.基金项目
国家自然科学基金资助项目(30070247). （30070247）
