重庆医学2012，Vol.41Issue(13)：1295-1296,封4,3.DOI:10.3969/j.issn.1671-8348.2012.13.020
烟熏联合脂多糖制备大鼠慢性阻塞性肺疾病动物模型
Establishment of rat chronic obstructive pulmonary disease model with cigarette inhalation and intratracheal instillation of LPS
摘要
Abstract
Objective To dynamically observe the pathogenic changes of lung tissue and small bronchia during constructing process of the rat model of chronic obstructive pulmonary disease(COPD) to further understand the developing process of COPD. Methods The COPD rat model was established by cigarette smoking inhalation for 4 weeks and twice intratracheal instillation of li-popolysaccharide(LPS). Then the model s lung tissues were taken on 7,14,21,28,42 d for pathological HE staining respectively. Results The COPD rat model on 28 d accorded with the pathomorphological characteristics of human COPD. The rats of COPD models gradually developed the manifestations of emphysema during the model-constructing process. Conclusion The stable COPD rat model may be successfully established by this combined methods of cigarette smoking inhalation and intratracheal instillation of LPS.关键词
肺疾病/慢性阻塞性/模型/动物/脂多糖类/烟草烟污染Key words
pulmonary disease,chronic obstructive/models,animal/lipopolysaccharides/tobacco smoke pollution引用本文复制引用
顾延会,欧阳瑶..烟熏联合脂多糖制备大鼠慢性阻塞性肺疾病动物模型[J].重庆医学,2012,41(13):1295-1296,封4,3.基金项目
贵州省科技厅社发关基金资助项目(黔科合SY字[2009]3053209.001.099.05). （黔科合SY字[2009]3053209.001.099.05）
