重庆医学2012，Vol.41Issue(13)：1299-1301,3.DOI:10.3969/j.issn.1671-8348.2012.13.022
荔枝核总黄酮对肝纤维化大鼠TGF-β1及Smad3、7表达的影响
Effect of total flavone of Litchi chinensis sonn on expression of TGF-β1,Smad3 and Smad7 of hepatic fibrosis in rats
摘要
Abstract
Objective To investigate the influence of total flavone(TFL)of Litchi Chinensis sonn on the expression of TGF-β1, Smad3 and Smad7 of hepatic fibrosis in rats,and to study the mechanism of TFL. Methods Seventy males SD rats were randomly divided into four groups:sham-operated group,model group,high-dose TFL group[200 mg/(kg · D)],low-dose group[l00 mg/(kg · d). The liver fibrosis model was prepared by bile duct ligation, where as the sham-operated group was used as controls. The rats were respectively killed at 2,3,4 weeks after operation. The liver histopathological changes were observed under light microscopy by HK staining. The expression and orientation of TGF-β1, Smad3 and Smad7 in hepatic fibrosis were detected by immunohisto-chemistry. Results TGF-β1 and Smad3 at 2,3,4 weeks in the sham-operated group were expressed in low amounts in the normal liver tissues, while Smad7 at the same points was decreased. Compared with the model group,the fibrotic degree of the liver tissues was reduced after high-dose TFL treatment. With the formation and development of fibrosis,the expression of TGF-β1 and Smad3 at 2,3 4 weeks were 3. 000 + 1. 309,2. 000 + 1. 309,1. 800 + 1. 649 and 2. 875 + 1. 458,2. 000 + 1. 309,1. 833 + 0. 753 respectively in the high-dose TFL group,while 3. 750 + 1.488,4. 333 + 1.211,5.000 + 0. 817 and 3. 375 + 0. 916,4.000 + 0.894,4. 250 + 0. 500 respectively in the model group,showing statistical difference between the two groups(P<0. 05). Conclusion TFL may effectively reduce the liver injury and hepatic fibrosis induced by common bile duct ligation. Its mechanism may inhibit the high expression of TGF-β1 and Smad3,and increase the expression of Smad7.关键词
荔枝核/黄酮/肝硬化/转化生长因子/Smad3蛋白质/Smad7蛋白质Key words
Semen Litchi/flavone/liver cirrhosis/transforming growth factors/Smad3 protein/Smad7 protein引用本文复制引用
刘 ,赵永忠,肖绪华,何志国..荔枝核总黄酮对肝纤维化大鼠TGF-β1及Smad3、7表达的影响[J].重庆医学,2012,41(13):1299-1301,3.基金项目
广西壮族自治区教育厅科研基金资助项目(201012MS172) （201012MS172）
广西壮族自治区卫生厅科研课题(22011169). （22011169）
