重庆医学2012，Vol.41Issue(14)：1353-1354,1357,3.DOI:10.3969/j.issn.1671-8348.2012.14.001
MALP-2对多形核中性细胞凋亡及其Bax蛋白表达的影响
Effects of MALP-2 on apoptosis and intracellular Bax expression of neutrophils
摘要
Abstract
To explore the effects of MALP-2 as TLR agonist on the apoptosis and intracellular Bax protein expression of polymorphonuclear neutrophils(PMN). Methods Heparinized peripheral blood was obtained from healthy volunteers. PMN were cultured according to experimental needs: the experimental group(MALP-2), positive control group(flagellin) and negative control group. Apoptosis of PMN was detected by flow cytometric analysis. The expression of Bax protein of PMN was examined by Western blotting analysis. Results Flow cytometry indicated that MALP-2 delayed PMN apoptosis. Western blotting showed that the expression of Bax protein in PMN was down-regulated by MALP-2. Conclusion MALP-2 might delay spontaneous apoptosis of PMN by reducing the expression of Bax protein in PMN.关键词
中性核细胞/细胞凋亡/Toll样受体/Bcl-2相关X蛋白质Key words
neutrophils/apoptosis/toll-like receptors/Bcl-2-associated X protein引用本文复制引用
高燕,叶迎春,袁青,周云刚,黄黎,刘佳佳..MALP-2对多形核中性细胞凋亡及其Bax蛋白表达的影响[J].重庆医学,2012,41(14):1353-1354,1357,3.基金项目
国家自然科学基金资助项目(30371305). （30371305）
