重庆医学2012，Vol.41Issue(14)：1394-1396,封3,4.DOI:10.3969/j.issn.1671-8348.2012.14.019
骨形态发生蛋白-2、梗死心肌裂解液对骨髓间充质干细胞体外诱导分化作用的研究
Study on role of bone morphogenetic protein 2,infarcted myocardium lysate on bone marrow mesenchymal stem cells induced in vitro differentiation
海澜 1高航2
作者信息
- 1. 辽宁医学院研究生院,辽宁锦州,121001
- 2. 辽宁医学院附属第一医院心内科,辽宁锦州,121001
- 折叠
摘要
Abstract
To investigate the influence of bone morphogenetic protein 2CBMP-2) ,infarcted myocardium lysate simulated biological microenvironment on bone marrow mesenchymal stem cells (BMSCs) differentiation into cardiomyocytes and the effect of BMP-2 on the induction in the simulation microenvironment. Methods BMSCs were separated and cultured in vitro. The rat model of myocardial infarction was constructed to prepare myocardial infarction lysate, which was divided into 4 groups: pure DMEM culture group(A) ,infarcted myocardium lysate group(B) ,infarcted myocardium lysate + BMP-2 blocker noggin group(C) and BMP-2 group(D). The morphological observation was performed by the inverted phase contrast microscope, the immunohisto-chemical technique was used for detecting the expression of cardiac specific troponin(cTnT) and myocardial specificity myosin heavy chain(MHC) expression,and the induced cardiomyocytes were analyzed by statistics. Results After 3-week induction,the immuno-cytochemistry analysis showed the negative expression of cTnT and MHC in the group A and C,and the positive expression in the group B and D. Comparing the group B and D with the group A and C,positive cells number was increased with statistical difference between them(P＜0. 05). Conclusion BMP-2 and infarcted myocardium lysate can induce the differentiation of BMSCs into cardiomyocytes and BMP-2 is one of important components during infarcted myocardium lysate induction process.关键词
骨髓祖代细胞/间质干细胞/肌细胞,心脏/骨形态发生蛋白质类/梗死心肌裂解液Key words
myeloid progenitor cells/mesenchymal stem cells/myocytes,cardiac/bone morphogenetic proteins/infarcted myocardium lysate引用本文复制引用
海澜,高航..骨形态发生蛋白-2、梗死心肌裂解液对骨髓间充质干细胞体外诱导分化作用的研究[J].重庆医学,2012,41(14):1394-1396,封3,4.
