南方医科大学学报2012，Vol.32Issue(1)：14-18,5.DOI:44-1627/R.20120101.1323.020
高糖降低内皮细胞ABCG1表达与内皮细胞损伤相关
Endothelial dysfunction induced by high glucose is associated with decreased ATP-binding cassette transporter G1 expression
摘要
Abstract
Objective To investigate the role of ATP-binding cassette transporter Gl (ABCG1) in endothelial dysfunction induced by high glucose. Methods Human aortic endothelial cells (HAECs) were incubated in the presence of 5.6 or 30 mmol/L glucose for 24-72 h with or without a 2-h pretreatment with the LXR agonist 22(R)-hydroxycholesterol. Real-time PCR and Western blotting were used to measure the mRNA and protein expressions of ABCG1; the intracellular cholesterol efflux and endothelial nitric oxide synthase (eNOS) activity were measured by scintillation counting. Results High glucose time-dependently suppressed ABCG1 expression and cholesterol efflux to HDL in HAECs. High glucose also decreased eNOS activity. ABCG1 down-regulation induced by high glucose, along with decreased cholesterol efflux and eNOS activity, was abolished by treatment of the cells with the LXR agonist. Conclusion Endothelial dysfunction induced by high glucose is associated with decreased ABCG1 expression.关键词
高糖/ABCG1/内皮细胞损伤/eNOS活性Key words
high glucose/ ATP-binding cassette transporter Gl/ endothelial dysfunction/ endothelial nitric oxide synthase分类
医药卫生引用本文复制引用
薛嘉虹,牛小麟,魏瑾,董新,朱参战,党寅虎,宋安齐,黄惠梅..高糖降低内皮细胞ABCG1表达与内皮细胞损伤相关[J].南方医科大学学报,2012,32(1):14-18,5.基金项目
国家自然科学基金(81100210,81170209) （81100210,81170209）
教育部博士点新教师基金(1896008) （1896008）
西安交通大学医学创新基金("高糖抑制细胞内脂质流出的作用机制探讨") （"高糖抑制细胞内脂质流出的作用机制探讨"）
