南方医科大学学报2012，Vol.32Issue(1)：28-31,4.DOI:44-1627/R.20111231.1545.001
25羟维生素D3对支气管上皮细胞维生素D受体表达及分布的影响
Effect of 25-hydroxyvitamin D3 on vitamin D receptor expression and distribution in human bronchial epithelial cells in vitro
摘要
Abstract
Objective To evaluate the effect of 25-hydroxyvitamin D5 on the expression and distribution of vitamin D receptor in normal human bronchial epithelial cells. Methods MTT assay was used to assess the "viability of human airway epithelial cell line 16HBE following a 24-h exposure to different concentrations of 25-hydroxyvitamin D3. Real-time quantitative PCR, Western blotting, and immunofluorescence assay were used to observe the expression and distribution of vitamin D receptor in the cells following the exposure. Results Compared with the control cells, 16HBE cells exposed to different concentrations of 25-hydroxyvitamin D3 exhibited no significantly increase in the expression or distribution of vitamin D receptor. Conclusion The influence of 25-hydroxyvitamin D3 on bronchial epithelial cells might be independent of the expression and translocation of vitamin D receptor.关键词
人气道上皮细胞/25羟维生素D3/维生素D受体Key words
human airway epithelium/ 25-hydroxy vitamin D3/ vitamin D receptor分类
医药卫生引用本文复制引用
董航明,赵海金,刘来昱,梁振宇,吕燕华,蔡绍曦..25羟维生素D3对支气管上皮细胞维生素D受体表达及分布的影响[J].南方医科大学学报,2012,32(1):28-31,4.基金项目
国家自然科学基金(30971328) （30971328）
教育部博士点基金(20094433110011) （20094433110011）
南方医院院长基金(20102002) （20102002）
