南方医科大学学报2012，Vol.32Issue(1)：61-65,5.DOI:44-1627/R.20111231.1652.004
两种方法提取的人血清对体外培养人真皮成纤维细胞促增殖作用的比较研究
Effect of human serum extracted by two different methods on human dermal fibroblast growth in vitro
摘要
Abstract
Objective To explore the optimal method for extracting human serum that retains rich growth factors. Methods Human serum was isolated by centrifugation of coagulated whole blood or by Anitua's method, and the concentrations of PDGF-AB and transforming growth factor-pi (TGF-f?l) in the serum samples were measured. Human dermal fibroblasts were cultured in the presence of 10% feral bovine serum or 10% human serum obtained, and the cell morphology, viability and proliferative activity of the cells were evaluated. Results The fibroblasts grew well in all the media with good viability. The cells cultured in the presence of human serum isolated by centrifugation of coagulated whole blood, which had the richest content of growth factors, showed the greatest cell number and cell viability among the groups (P<0.05), a result consistent with the growth curve and MTT curve. Conclusion Centrifugation of coagulated whole blood retains high contents of growth factors in human serum to better promote cell growth, and is simple, cost-effective and most efficient for serum isolation.关键词
人血清/人真皮成纤维细胞/生长因子/提取方法/细胞培养Key words
human serum/ human dermal fibroblasts/ growth factors/ extract methods/ cell culture分类
医药卫生引用本文复制引用
鲁华,鲁峰,刘戈,高建华..两种方法提取的人血清对体外培养人真皮成纤维细胞促增殖作用的比较研究[J].南方医科大学学报,2012,32(1):61-65,5.基金项目
国家自然科学基金(30901566,81071589) （30901566,81071589）
广东省自然科学基金(S2011010003058) （S2011010003058）
