南方医科大学学报2012，Vol.32Issue(1)：125-127,3.DOI:44-1627/R.20120101.1246.015
高效液相色谱—质谱法测定泌尿系结石中的三聚氰胺
Determination of melamine by high-performance liquid chromatography-mass spectrometry in urinary calculi
摘要
Abstract
Objective To establish a high-performance liquid chromatography-mass spectrometry (LC//MS)-based method for efficient determination of melamine in urinary calculi in children taking melamine-contaminated formula milk powder. Methods LC/MS was employed to determine the contents of melamine in urinary calculi surgically removed from 17 children with a history of taking melamine-contaminated milk powder and in 4 samples of uric acid stone from adults. The positive ionization mode of electro-spray ionization source was used, and the limit of melamine determination was 0.1 mg/kg. Results No melamine was detected in the 4 uric acid stone samples from adults. Melamine was detected in 4 samples of urinary calculi from the 17 children, with the concentration ranging from 0.8 to 64 mg/kg. Conclusion LC/MS is simple and effective for detecting melamine in urinary calculi, which is helpful to the treatment and follow-up.关键词
三聚氰胺/泌尿系结石/儿童/高效液相色谱—质潜法Key words
melamine/ urolithiasis/ children/ high-performance liquid chromatography-mass spectrometry分类
医药卫生引用本文复制引用
吴文起,王露萍,欧莉莉,梁叶萍,麦赞林,李舒珏,单炽昌,袁坚,曾国华..高效液相色谱—质谱法测定泌尿系结石中的三聚氰胺[J].南方医科大学学报,2012,32(1):125-127,3.基金项目
广州市科技计划项目(2009J1-C371-2) （2009J1-C371-2）
