南方医科大学学报2012，Vol.32Issue(2)：173-176,4.DOI:44-1627/R.20120209.1202.006
胆管结扎诱导的肝纤维化大鼠肝脏上皮间质表型的变化
Epithelial to mesenchymal phenotype transition in the liver of rats with bile duct ligation
摘要
Abstract
Objective To investigate the occurrence of epithelial to mesenchymal phenotype transition (EMT) in the liver of rats following bile duct ligation (BDL). Methods Twenty-four male Wistar rats were randomized into sham-operated group and BDL group. Liver fibrosis of the rats was evaluated by HE staining and Masson's trichrome staining. Western blotting was used to detect the expression levels of the epithelial markers albumin and E-cadherin and the mesenchymal markers type I collagen and vimentin in the liver tissue. Immunofluorescence was employed to determine the co-localizations of FSP-1 + vimentin, FSP-1+type I collagen, FSP-1+albumin, and albumin+type I collagen in cells. Results Compared with those in sham-operated group, the rats in BDL group showed significantly increased ISHAK fibrosis score (4.42±1.16 vs 0, P=0.000), METAVIR fibrosis score (3.42±0.67 vs 0, P=0.000) and type I collagen levels (0.30±0.06 vs 0.11±0.07, P=0.000) with up-regulated protein levels of albumin (0.53±0.63 vs 1.12±0.01, P=0.000) and E-cadherin (0.21±0.01 vs 0.44±0.01, P=0.000) and down-regulated type I collagen (8.21 ±0.12 vs 0.24±0.01, P=0.000) and vimentin (3.14±0.01 vs 0.37±0.01, P=0.000). The number of cells with co-localizations of FSP-1+vimentin, FSP-1+type I collagen, FSP-1+albumin, and albumin+type I collagen was also significantly increased in BDL group. Conclusion BDL causes significantly decreased expression of epithelial markers and increased expressions of the mesenchymal markers in rats, indicating the occurrence of EMT in some of the liver cells.关键词
上皮-间质表型转化/肝纤维化/Ⅰ型胶原/METAVIR纤维化评分Key words
epithelial mesenchymal transition/ hepatic fibrosis/ type I collagen/ METAVTR fibrosis score分类
医药卫生引用本文复制引用
黄珊,张文雍,潘春球,罗薇,余常辉,孟莹,李旭..胆管结扎诱导的肝纤维化大鼠肝脏上皮间质表型的变化[J].南方医科大学学报,2012,32(2):173-176,4.基金项目
国家自然科学基金(81070338) （81070338）
广东省科技计划项目(2010B060500008) （2010B060500008）
王宝恩肝纤维化科研基金(20030018) （20030018）
