南方医科大学学报2012，Vol.32Issue(2)：189-192,4.DOI:44-1627/R.20120209.1417.019
丁酸钠对人结肠癌细胞株HCT-116细胞凋亡及基质相互作用分子和Orai1蛋白活性的影响
Effect of sodium butyrate on apoptosis and stromal interaction molecule and Orai1 activity in human colon cancer HCT-116 cells in vitro
摘要
Abstract
Objective To investigate the mechanism underlying sodium butyrate (NaB)-induced apoptosis of a human colon cancer cell line HCT-116. Methods The apoptosis of HCT-116 cells induced by NaB was confirmed by hoechst33342 staining and AnnexinV+ PI assay. The changes in the intracellular localization of stromal interaction molecule (STTM1) and Orail following NaB treatment were detected by immunofluorescence technique. Western blotting was used to investigate the protein expression levels of STIM1 and Orail. Results NaB induced apoptosis and caused translocation and colocalization of STIM1 and Orail in HCT-116 cells. Conclusion The apoptosis of human colon cancer cells induced by NaB is correlated to the redistribution of STTM1 and Orail.关键词
丁酸钠/人结肠癌细胞/细胞凋亡/STIM1/Orai1Key words
sodium butyrate/ human colon cancer cells/ apoptosis/ stromal interaction molecule/ Orail分类
医药卫生引用本文复制引用
孙素霞,李文军,陈思强,张贺,余少珍,张敏红,邹飞..丁酸钠对人结肠癌细胞株HCT-116细胞凋亡及基质相互作用分子和Orai1蛋白活性的影响[J].南方医科大学学报,2012,32(2):189-192,4.基金项目
国家自然科学基金(30971193) （30971193）
